| 质粒名称: | pMK381 (AAVS1 CMV-OsTIR1F74G) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140536 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pMK359 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CMV-OsTIR1(F74G) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMK381 (AAVS1 CMV-OsTIR1F74G) was a gift from Masato Kanemaki (Addgene plasmid # 140536 ; http://n2t.net/addgene:140536 ; RRID:Addgene_140536) |
| 参考文献部分: | The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT.. Nat Commun (2020) 11(1):5701 10.1038/s41467-020-19532-z |
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pMK381 (AAVS1 CMV-OsTIR1F74G)载体质粒基本信息
pMK381 (AAVS1 CMV-OsTIR1F74G)质粒图谱载体图谱和pMK381 (AAVS1 CMV-OsTIR1F74G)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pMK381 (AAVS1 CMV-OsTIR1F74G)质粒载体简介
pMK381 (AAVS1 CMV-OsTIR1F74G)质粒序列载体序列
LOCUS pMK381 (AAVS1 CMV-OsTIR1F74G) 10098 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION AAVS1 CMV-OsTIR1(F74G).
ACCESSION .
VERSION .
KEYWORDS pMK381 (AAVS1 CMV-OsTIR1F74G)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10098)
AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano
R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y,
Hayashi KI, Kanemaki MT.
TITLE The auxin-inducible degron 2 technology provides sharp degradation
control in yeast, mammalian cells, and mice.
JOURNAL Nat Commun (2020) 11(1):5701
REFERENCE 2 (bases 1 to 10098)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10098
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 295..1098
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
enhancer 1153..1456
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1457..1660
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1611..1631
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
polyA_signal 3599..3720
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3636..3655)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3690..3709
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind complement(3747..3780)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 3841..4340
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(3876..3899)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4273..4292
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 4380..4979
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4380..4399)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
protein_bind complement(5536..5569)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5600..6436
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(6470..6489)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6471..6489)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6496..6513)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6496..6512)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6505..6527)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 6650..6952
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6831..6850
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS 7301..8095
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7355..7374)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7965..7984
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS complement(8345..9205)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8968..8987
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9329..9917
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9818..9837
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10071..10088
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
1081 tccaccccac agtggggcaa gctagtaatc aattacgggg tcattagttc atagcccata
1141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
1201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
1261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
1321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
1381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
1441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
1501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
1561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
1621 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
1681 ccgctagcgc tgccaccatg acatactttc ctgaagaggt cgtcgaacac atttttagct
1741 tcctgcctgc acagagagat agaaacacag tgagcctggt ctgcaaagtg tggtacgaga
1801 tcgaacgcct gagccggaga ggagtgttcg tcggcaactg ctatgctgtg agagcaggca
1861 gggtcgccgc taggtttcca aatgtgcgcg cactgaccgt caaggggaaa ccccacggcg
1921 ccgactttaa cctggtgccc cctgattggg gaggatacgc cggcccttgg atcgaggcag
1981 ccgctcgcgg ctgtcatgga ctggaggaac tgcgcatgaa gcgaatggtg gtctctgacg
2041 aaagtctgga gctgctggct cggagcttcc ctaggtttcg cgcactggtg ctgatttctt
2101 gcgaaggctt cagcaccgat ggactggcag ccgtggcctc ccactgtaag ctgctgcggg
2161 agctggacct ccaggagaat gaagtggagg atagaggccc cagatggctg tcttgcttcc
2221 cagactcatg taccagcctg gtgtccctga actttgcctg catcaaaggc gaagtgaatg
2281 ctgggtccct ggagcggctg gtctcaagaa gccccaacct gaggtctctg cggctgaacc
2341 ggagcgtgag cgtggacact ctggctaaga ttctgctgag aacccctaac ctggaggatc
2401 tgggaaccgg caatctgaca gacgatttcc agacagaatc ctactttaaa ctgacttctg
2461 ccctggagaa gtgtaaaatg ctgaggagtc tgtcaggatt ctgggatgct tcacccgtgt
2521 gcctgagctt tatctaccct ctgtgtgcac agctgacagg cctgaacctg agctatgcac
2581 caaccctgga cgccagtgat ctgacaaaga tgatctcacg ctgcgtgaaa ctccagcgac
2641 tgtgggtgct ggactgtatt tccgataagg ggctccaggt ggtcgccagc tcctgcaagg
2701 acctccagga gctgagagtg ttcccatctg atttttacgt ggccggatat agtgctgtca
2761 ctgaggaagg cctggtggca gtctcactgg gatgcccaaa gctgaacagc ctgctgtatt
2821 tctgtcatca gatgactaat gctgcactgg tgaccgtcgc caagaactgc cctaatttca
2881 cccgatttcg gctgtgtatt ctggaaccag gcaaacccga cgtggtcaca tcccagccac
2941 tggatgaagg gtttggagct atcgtgagag agtgcaaggg actccagagg ctgagcattt
3001 ccggcctgct gacagacaaa gtgttcatgt acatcggcaa gtatgctaag cagctggaga
3061 tgctgagcat tgcatttgcc ggagactccg ataagggcat gatgcacgtg atgaacgggt
3121 gtaagaatct gcgaaaactg gaaatccggg acagcccttt cggggatgcc gctctgctgg
3181 gaaactttgc cagatacgag acaatgagga gcctgtggat gtctagttgc aatgtgactc
3241 tgaagggctg tcaggtcctg gctagtaaaa tgcctatgct gaacgtggaa gtcattaatg
3301 agcgggacgg gtctaacgaa atggaggaaa atcatggcga cctgccaaag gtggagaaac
3361 tgtatgtgta tcggaccacc gcaggggcaa gagatgatgc tcccaacttt gtgaagattc
3421 tgtgaggatc catcggatcc cgggcccgtc gacggtaccg cgggcccggg atccaccggg
3481 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
3541 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
3601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
3661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
3721 agcgatcata ttcaataacc cttaatataa cttcgtataa tgtatgctat acgaagttat
3781 taggtctgaa gaggagttta cgtccagcca agcttaggat ctcgacctcg aaattctacc
3841 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
3901 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
3961 gccaaccgac tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
4021 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
4081 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
4141 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
4201 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
4261 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
4321 cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca
4381 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
4441 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
4501 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
4561 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
4621 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
4681 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
4741 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
4801 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
4861 agacctccgc gccccacaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
4921 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
4981 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatga
5041 tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat gtccactaaa
5101 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
5161 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct
5221 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
5281 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcta
5341 tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt ctaagtactg
5401 tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc tctgttccac
5461 atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag ctggtcgaga
5521 tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
5581 aagaggttca ctaagcttta ctagggacag gattggtgac agaaaagccc catccttagg
5641 cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc
5701 cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa
5761 ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga
5821 gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc
5881 cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag
5941 cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt
6001 cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg
6061 cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg
6121 aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca
6181 ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc
6241 cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc
6301 tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct
6361 gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg
6421 ttcctggctc tgctctaagg gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga
6481 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
6541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
6601 ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt
6661 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
6721 tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
6781 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
6841 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
6901 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
6961 catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
7021 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
7081 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
7141 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
7201 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
7261 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
7321 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
7381 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
7441 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
7501 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
7561 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
7621 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
7681 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
7741 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
7801 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
7861 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
7921 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
7981 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
8041 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
8101 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
8161 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
8221 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc
8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
8641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
8701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
8761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
8821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
8881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
8941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
9001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
9061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
9121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
9181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
9241 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
9301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
9361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
9421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
9481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
9541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
9601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
9661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
9721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
9781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
9841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
9901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
9961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
10021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
10081 tgagcgagga agcggaag
//
